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Protocol for Quantifying Zinc Flux in Cultured Cells using Fluorescent Indicators

Zinc (Zn(2+)) plays a vital role in the functioning of the cell. Cells have influx and efflux zinc transporters to regulate the levels of Zn(2+) in the cytoplasm and organellar compartments to maintain homeostasis. We present a protocol to measure changes in cellular zinc concentrations using either...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Ali, Saima, Cuajungco, Math P.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2020
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580219/
https://ncbi.nlm.nih.gov/pubmed/33111096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100050
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