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Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose Gradient

Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tis...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Tarazón, Estefanía, Portolés, Manuel, Roselló-Lletí, Esther
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580119/
https://ncbi.nlm.nih.gov/pubmed/33111127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100100
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