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Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging

Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular an...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Tripathi, Madhulika, Yen, Paul Michael, Singh, Brijesh Kumar
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580094/
https://ncbi.nlm.nih.gov/pubmed/33111102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100064
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