CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells

The CRISPR/Cas9 system is a powerful tool to generate a specific loss-of-function phenotype by gene knockout (KO). However, this approach is challenging in primary human cells. In this technical report, we present a reliable protocol to achieve a functional KO in the genome of human adipose stem/pro...

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Bibliografische gegevens
Gepubliceerd in:Adipocyte
Hoofdauteurs: Mandl, Markus, Ritthammer, Heike, Ejaz, Asim, Wagner, Sonja A., Hatzmann, Florian M., Baumgarten, Saphira, Viertler, Hans P., Zwierzina, Marit E., Mattesich, Monika, Schiller, Valerie, Rauchenwald, Tina, Ploner, Christian, Waldegger, Petra, Pierer, Gerhard, Zwerschke, Werner
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Taylor & Francis 2020
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Research Paper
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7575003/
https://ncbi.nlm.nih.gov/pubmed/33070670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21623945.2020.1834230
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