Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions

[Image: see text] The formation of cytomimetic protocells that capture the physicochemical aspects of living cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded cytoplasm in liposome-based protocells and studied the kinetics of cell-free gene expression in these...

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Udgivet i:ACS Synth Biol
Main Authors: Vibhute, Mahesh A., Schaap, Mark H., Maas, Roel J. M., Nelissen, Frank H. T., Spruijt, Evan, Heus, Hans A., Hansen, Maike M. K., Huck, Wilhelm T. S.
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2020
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7573978/
https://ncbi.nlm.nih.gov/pubmed/32976714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.0c00330
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