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Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2

To understand how the RuvC catalytic domain of Class 2 Cas proteins cleaves DNA, it will be necessary to elucidate the structures of RuvC-containing Cas complexes in their catalytically competent states. Cas12i2 is a Class 2 type V-I CRISPR-Cas endonuclease that cleaves target dsDNA by an unknown me...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Huang, Xue, Sun, Wei, Cheng, Zhi, Chen, Minxuan, Li, Xueyan, Wang, Jiuyu, Sheng, Gang, Gong, Weimin, Wang, Yanli
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7567891/
https://ncbi.nlm.nih.gov/pubmed/33067443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-19072-6
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