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Dictyostelium Cell Fixation: Two Simple Tricks

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context,...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Methods Protoc
Prif Awduron: Koonce, Michael, Tikhonenko, Irina, Gräf, Ralph
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MDPI 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7565140/
https://ncbi.nlm.nih.gov/pubmed/32630359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3030047
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