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Dictyostelium Cell Fixation: Two Simple Tricks

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context,...

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Detalhes bibliográficos
Publicado no:Methods Protoc
Main Authors: Koonce, Michael, Tikhonenko, Irina, Gräf, Ralph
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7565140/
https://ncbi.nlm.nih.gov/pubmed/32630359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3030047
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