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Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA

CRISPR-based diagnostics (CRISPR-Dx) has shown great promise in molecular diagnostics, but its utility in the sensing of microRNA (miRNA) biomarkers is limited by sensitivity, cost and robustness. Here, we describe a CRISPR-Dx method for the sensitive and cost-effective detection of miRNAs by ration...

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Vydáno v:Chem Sci
Hlavní autoři: Peng, Shuang, Tan, Zhen, Chen, Siyu, Lei, Chunyang, Nie, Zhou
Médium: Artigo
Jazyk:Inglês
Vydáno: Royal Society of Chemistry 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7553042/
https://ncbi.nlm.nih.gov/pubmed/33133487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0sc03084h
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