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A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes

Hepatitis B virus (HBV) is a para-retrovirus that reverse transcribes its pregenomic RNA into relaxed circular DNA inside viral nucleocapsids. The number of HBV genomes produced in vitro is typically quantified using commercial silica-membrane-based nucleic acid purification kits to isolate total DN...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Viruses
Hauptverfasser: Jo, Eunji, Yang, Jaewon, Koenig, Alexander, Yoon, Seung Kew, Windisch, Marc P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7552047/
https://ncbi.nlm.nih.gov/pubmed/32846983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v12090928
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