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A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA puri...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3983595/ https://ncbi.nlm.nih.gov/pubmed/24722424 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04659 |
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