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Expansion of mouse primitive hematopoietic cells in three-dimensional cultures on chemically fixed stromal cell layers

To establish a practical and convenient method to expand hematopoietic cells (HCs), we applied chemically-fixed stromal cell layers formed within three-dimensional (3D) scaffolds to feeder of HC cultures. The HCs were expanded using two successive cultures. First, stromal cells were cultured within...

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Detalhes bibliográficos
Publicado no:Cytotechnology
Main Authors: Miyoshi, Hirotoshi, Shimizu, Yuichiro, Yasui, Yutaka, Sugiyama, Satoshi
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7547917/
https://ncbi.nlm.nih.gov/pubmed/32897481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-020-00417-4
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