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Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies
AAV-CRISPR/Cas9 permits gene mutagenesis in the adult CNS. Current methods determining in vivo on-target mutagenesis have been limited by the ability to isolate virally transduced cells. This protocol optimizes a workflow for the design, cloning, and validation of sgRNAs delivered by AAVs in vivo th...
Guardat en:
| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7544006/ https://ncbi.nlm.nih.gov/pubmed/33043306 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100070 |
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