Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato

Genome editing via the homology‐directed repair (HDR) pathway in somatic plant cells is very inefficient compared with error‐prone repair by nonhomologous end joining (NHEJ). Here, we increased HDR‐based genome editing efficiency approximately threefold compared with a Cas9‐based single‐replicon sys...

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Sparad:
Bibliografiska uppgifter
I publikationen:Plant Biotechnol J
Huvudupphovsmän: Vu, Tien Van, Sivankalyani, Velu, Kim, Eun‐Jung, Doan, Duong Thi Hai, Tran, Mil Thi, Kim, Jihae, Sung, Yeon Woo, Park, Minwoo, Kang, Yang Jae, Kim, Jae‐Yean
Materialtyp: Artigo
Språk:Inglês
Publicerad: John Wiley and Sons Inc. 2020
Ämnen:
Research Articles
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7540044/
https://ncbi.nlm.nih.gov/pubmed/32176419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.13373
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