Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato

Genome editing via the homology‐directed repair (HDR) pathway in somatic plant cells is very inefficient compared with error‐prone repair by nonhomologous end joining (NHEJ). Here, we increased HDR‐based genome editing efficiency approximately threefold compared with a Cas9‐based single‐replicon sys...

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Detalhes bibliográficos
Publicado no:Plant Biotechnol J
Main Authors: Vu, Tien Van, Sivankalyani, Velu, Kim, Eun‐Jung, Doan, Duong Thi Hai, Tran, Mil Thi, Kim, Jihae, Sung, Yeon Woo, Park, Minwoo, Kang, Yang Jae, Kim, Jae‐Yean
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7540044/
https://ncbi.nlm.nih.gov/pubmed/32176419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.13373
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