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Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis

A large number of aggressive cancer cell lines display elevated levels of activated Rac1, a small GTPase widely implicated in cytoskeleton reorganization, cell motility, and metastatic dissemination. A commonly accepted methodological approach for detecting Rac1 activation in cancer cells involves t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Biol Chem
Hauptverfasser: Baker, Martin J., Cooke, Mariana, Kreider-Letterman, Gabriel, Garcia-Mata, Rafael, Janmey, Paul A., Kazanietz, Marcelo G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7535912/
https://ncbi.nlm.nih.gov/pubmed/32817335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.013919
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