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Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans

Precision genome editing for model organisms has revolutionized functional analysis and validation of a wide variety of molecular systems. To date, the capacity to insert single-copy transgenes into the model nematode Caenorhabditis elegans has focused on utilizing either transposable elements or CR...

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Bibliographische Detailangaben
Veröffentlicht in:G3 (Bethesda)
Hauptverfasser: Stevenson, Zachary C., Moerdyk-Schauwecker, Megan J., Jamison, Brennen, Phillips, Patrick C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Genetics Society of America 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7534419/
https://ncbi.nlm.nih.gov/pubmed/32816924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.120.401400
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