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An optimized step‐by‐step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse intervertebral discs and subsequent preparation of high‐quality intact total RNA

Intervertebral disc degeneration is the most significant, and least understood, cause of chronic back pain, affecting almost one in seven individuals at some point of time. Each intervertebral disc has three components; central nucleus pulposus (NP), concentric layers of annulus fibrosus (AF), and a...

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Detaylı Bibliyografya
Yayımlandı:JOR Spine
Asıl Yazarlar: Piprode, Vikrant, Mohanty, Sarthak, Bonavita, Raffaella, Loh, Sarah, Anbazhagan, Rajakumar, Saini, Chandan, Pinelli, Robert, Pricop, Paul, Dahia, Chitra L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: John Wiley & Sons, Inc. 2020
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7524240/
https://ncbi.nlm.nih.gov/pubmed/33015579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jsp2.1108
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