An optimized step‐by‐step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse intervertebral discs and subsequent preparation of high‐quality intact total RNA

Intervertebral disc degeneration is the most significant, and least understood, cause of chronic back pain, affecting almost one in seven individuals at some point of time. Each intervertebral disc has three components; central nucleus pulposus (NP), concentric layers of annulus fibrosus (AF), and a...

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Bibliographic Details
Published in:JOR Spine
Main Authors: Piprode, Vikrant, Mohanty, Sarthak, Bonavita, Raffaella, Loh, Sarah, Anbazhagan, Rajakumar, Saini, Chandan, Pinelli, Robert, Pricop, Paul, Dahia, Chitra L.
Format: Artigo
Language:Inglês
Published: John Wiley & Sons, Inc. 2020
Subjects:
Protocols, Methods, and Resources
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7524240/
https://ncbi.nlm.nih.gov/pubmed/33015579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jsp2.1108
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