An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro

RNA-binding proteins are key regulators of cell identity and function, which underscores the need for unbiased and versatile protocols to identify and characterize novel protein-RNA interactions. Here, we describe a simple and cost-effective in vitro RNA immunoprecipitation (iv-RIP) method to assess...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:STAR Protoc
Main Authors: Fuentes-Iglesias, Alejandro, Garcia-Outeiral, Vera, Pardavila, Jose Angel, Wang, Jianlong, Fidalgo, Miguel, Guallar, Diana
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2020
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7521669/
https://ncbi.nlm.nih.gov/pubmed/32995755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100093
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