Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native c...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Timón-Gómez, Alba, Pérez-Pérez, Rafael, Nyvltova, Eva, Ugalde, Cristina, Fontanesi, Flavia, Barrientos, Antoni
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7521667/
https://ncbi.nlm.nih.gov/pubmed/32995753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100089
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