Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native c...

Full beskrivning

Sparad:
Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Timón-Gómez, Alba, Pérez-Pérez, Rafael, Nyvltova, Eva, Ugalde, Cristina, Fontanesi, Flavia, Barrientos, Antoni
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2020
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7521667/
https://ncbi.nlm.nih.gov/pubmed/32995753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100089
Taggar: Lägg till en tagg
Inga taggar, Lägg till första taggen!

Liknande verk