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Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655
Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1...
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| Publicado no: | AMB Express |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Berlin Heidelberg
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7519928/ https://ncbi.nlm.nih.gov/pubmed/32979149 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-020-01099-z |
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