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pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transc...

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Detalhes bibliográficos
Publicado no:Microbiol Resour Announc
Main Authors: Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, Kalinowski, Jörn
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7516140/
https://ncbi.nlm.nih.gov/pubmed/32972929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MRA.00596-20
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