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SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing
Selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (m...
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| Pubblicato in: | Methods Mol Biol |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7510887/ https://ncbi.nlm.nih.gov/pubmed/32712920 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0716-9_10 |
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