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SHAPE Profiling to Probe Group II Intron Conformational Dynamics During Splicing

Selective 2’-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (m...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Wiryaman, Timothy, Toor, Navtej
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7510887/
https://ncbi.nlm.nih.gov/pubmed/32712920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0716-9_10
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