Engineering Burkholderia xenovorans LB400 BphA through Site-Directed Mutagenesis at Position 283

Biphenyl dioxygenase (BPDO), which is a Rieske-type oxygenase (RO), catalyzes the initial dioxygenation of biphenyl and some polychlorinated biphenyls (PCBs). In order to enhance the degradation ability of BPDO in terms of a broader substrate range, the BphAE(S283M), BphAE(p4-S283M), and BphAE(RR41-...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Li, Junde, Min, Jun, Wang, Yuan, Chen, Weiwei, Kong, Yachao, Guo, Tianyu, Mahto, Jai Krishna, Sylvestre, Michel, Hu, Xiaoke
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2020
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7499034/
https://ncbi.nlm.nih.gov/pubmed/32709719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01040-20
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