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Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations

We describe the construction, expression and purification of three new membrane scaffold proteins (MSP) for use in assembling Nanodiscs. These new MSPs have a variety of luminescent properties for use in combination with several analytical methods. “Dark” MSP has no tryptophan residues, “Ultra-Dark”...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Anal Biochem
Main Authors: McLean, Mark A., Denisov, Ilia G., Grinkova, Yelena V., Sligar, Stephen G.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7492454/
https://ncbi.nlm.nih.gov/pubmed/32750355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2020.113860
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