Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Gene-targeted animal models that are generated by injecting Cas9 and sgRNAs into zygotes are often accompanied by undesired double-strand break (DSB)-induced byproducts and random biallelic targeting due to uncontrollable Cas9 targeting activity. Here, we establish a parental allele-specific gene-ta...

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Izdano u:Nat Commun
Glavni autori: Li, Yanhe, Weng, Yuteng, Bai, Dandan, Jia, Yanping, Liu, Yingdong, Zhang, Yalin, Kou, Xiaochen, Zhao, Yanhong, Ruan, Jingling, Chen, Jiayu, Yin, Jiqing, Wang, Hong, Teng, Xiaoming, Wang, Zuolin, Liu, Wenqiang, Gao, Shaorong
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2020
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Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7490392/
https://ncbi.nlm.nih.gov/pubmed/32929070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-18391-y
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