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Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins
We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in nuclear speckles...
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| Publicado en: | J Cell Biol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Rockefeller University Press
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7480118/ https://ncbi.nlm.nih.gov/pubmed/32609799 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201910207 |
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