A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic research a...

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Pubblicato in:mSphere
Autori principali: Shema Mugisha, Christian, Vuong, Hung R., Puray-Chavez, Maritza, Bailey, Adam L., Fox, Julie M., Chen, Rita E., Wessel, Alex W., Scott, Jason M., Harastani, Houda H., Boon, Adrianus C. M., Shin, Haina, Kutluay, Sebla B.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2020
Soggetti:
Observation
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7471006/
https://ncbi.nlm.nih.gov/pubmed/32878932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00658-20
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