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Application of different types of CRISPR/Cas-based systems in bacteria
As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria. However, the intrinsic toxicity of Cas9 and Cas12a-mediated CRISPR/Cas tools can lead to cell death in some strains, whic...
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| Publicado no: | Microb Cell Fact |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7470686/ https://ncbi.nlm.nih.gov/pubmed/32883277 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01431-z |
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