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Using Fluorescence Detected Sedimentation Velocity for Studying High Affinity Protein Interactions
Sedimentation velocity (SV) analytical ultracentrifugation is a classical technique for the real-time observation of macromolecular migration free in solution driven by centrifugal force. This enables the analysis of macromolecular mass, shape, size-distribution, and interactions. While traditionall...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7466938/ https://ncbi.nlm.nih.gov/pubmed/28771239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.064 |
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