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Using Fluorescence Detected Sedimentation Velocity for Studying High Affinity Protein Interactions

Sedimentation velocity (SV) analytical ultracentrifugation is a classical technique for the real-time observation of macromolecular migration free in solution driven by centrifugal force. This enables the analysis of macromolecular mass, shape, size-distribution, and interactions. While traditionall...

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Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Chaturvedi, Sumit K., Ma, Jia, Zhao, Huaying, Schuck, Peter
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7466938/
https://ncbi.nlm.nih.gov/pubmed/28771239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.064
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