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Genetically encoding thyronine for fluorescent detection of peroxynitrite

Peroxynitrite is a highly reactive oxidant effecting cell signaling and cell death. Here we report a fluorescent protein probe to selectively detect peroxynitrite. A novel unnatural amino acid, thyronine (Thy), was genetically encoded in E. coli and mammalian cells by evolving an orthogonal tRNA(Pyl...

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書目詳細資料
發表在:Bioorg Med Chem
Main Authors: Li, Shanshan, Yang, Bing, Kobayashi, Tomonori, Yu, Bingchen, Liu, Jun, Wang, Lei
格式: Artigo
語言:Inglês
出版: 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7461609/
https://ncbi.nlm.nih.gov/pubmed/32828428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2020.115665
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