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Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors

It is widely understood that CRISPR-Cas9 technology is revolutionary, with well-recognized issues including the potential for off-target edits and the attendant need for spatiotemporal control of editing. Here, we describe a far-red light (FRL)–activated split-Cas9 (FAST) system that can robustly in...

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Bibliographische Detailangaben
Veröffentlicht in:Sci Adv
Hauptverfasser: Yu, Yuanhuan, Wu, Xin, Guan, Ningzi, Shao, Jiawei, Li, Huiying, Chen, Yuxuan, Ping, Yuan, Li, Dali, Ye, Haifeng
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Association for the Advancement of Science 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7455487/
https://ncbi.nlm.nih.gov/pubmed/32923591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.abb1777
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