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Structure‐guided evolution of Green2 toward photostability and quantum yield enhancement by F145Y substitution

Quantum yield is a determinant for fluorescent protein (FP) applications and enhancing FP brightness through gene engineering is still a challenge. Green2, our de novo FP synthesized by microfluidic picoarray and cloning, has a significantly lower quantum yield than enhanced green FP, though they ha...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Protein Sci
Prif Awduron: Sun, Tingting, Li, Tianpeng, Yi, Ke, Gao, Xiaolian
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: John Wiley & Sons, Inc. 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7454421/
https://ncbi.nlm.nih.gov/pubmed/32715541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3917
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