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Low-Background Acyl-Biotinyl Exchange Largely Eliminates the Coisolation of Non-S-Acylated Proteins and Enables Deep S-Acylproteomic Analysis

Protein S-acylation (also called palmitoylation) is a common post-translational modification whose deregulation plays a key role in the pathogenesis of many diseases. Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of S-acylated proteins, has the potential of capturing the enti...

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Vydáno v:Anal Chem
Hlavní autoři: Zhou, Bo, Wang, Yang, Yan, Yiwu, Mariscal, Javier, Di Vizio, Dolores, Freeman, Michael R., Yang, Wei
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7451198/
https://ncbi.nlm.nih.gov/pubmed/31251020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b01520
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