Two-color nanoscopy of organelles for extended times with HIDE probes

Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables two-color super-resolution (STED) and 3D confocal imaging of two organelles...

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Udgivet i:Nat Commun
Main Authors: Chu, Ling, Tyson, Jonathan, Shaw, Juliana E., Rivera-Molina, Felix, Koleske, Anthony J., Schepartz, Alanna, Toomre, Derek K.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7450044/
https://ncbi.nlm.nih.gov/pubmed/32848153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-17859-1
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