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Fused Split Inteins: Tools for Introducing Multiple Protein Modifications
The split inteins from the DnaE cyanobacterial family are efficient and versatile tools for protein engineering and chemical biology applications. Their ultrafast splicing kinetics allow the efficient production of native proteins from two separate polypeptides both in vitro and in cells. They can a...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7442422/ https://ncbi.nlm.nih.gov/pubmed/32144667 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0434-2_8 |
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