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Fused Split Inteins: Tools for Introducing Multiple Protein Modifications

The split inteins from the DnaE cyanobacterial family are efficient and versatile tools for protein engineering and chemical biology applications. Their ultrafast splicing kinetics allow the efficient production of native proteins from two separate polypeptides both in vitro and in cells. They can a...

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Bibliografische gegevens
Gepubliceerd in:Methods Mol Biol
Hoofdauteurs: Lim, Byung Joon, Berkeley, Raymond F., Debelouchina, Galia T.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7442422/
https://ncbi.nlm.nih.gov/pubmed/32144667
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0434-2_8
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