Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition

Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively fused to an intein N-terminal fragme...

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Veröffentlicht in:Nat Commun
Hauptverfasser: Yao, Zhong, Aboualizadeh, Farzaneh, Kroll, Jason, Akula, Indira, Snider, Jamie, Lyakisheva, Anna, Tang, Priscilla, Kotlyar, Max, Jurisica, Igor, Boxem, Mike, Stagljar, Igor
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7229206/
https://ncbi.nlm.nih.gov/pubmed/32415080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-16299-1
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