A Modified Pre-plating Method for High-Yield and High-Purity Muscle Stem Cell Isolation From Human/Mouse Skeletal Muscle Tissues

Primary culture of skeletal muscle stem cells (MuSCs) is indispensable to study the dynamics of muscle regeneration and homeostasis. Here we describe the modified pre-plating method for isolating MuSCs in culture with greatly improved purity, yield, and procedure time. The protocol is based on the d...

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Detalhes bibliográficos
Publicado no:Front Cell Dev Biol
Main Authors: Yoshioka, Kiyoshi, Kitajima, Yasuo, Okazaki, Narihiro, Chiba, Ko, Yonekura, Akihiko, Ono, Yusuke
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Cell and Developmental Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7438441/
https://ncbi.nlm.nih.gov/pubmed/32903486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2020.00793
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