Genome editing in PDS genes of tomatoes by non-selection method and of Nicotiana benthamiana by one single guide RNA to edit two orthologs

The CRISPR/Cas9 system is widely used for targeted mutagenesis in many organisms including plants. For application of this system, tissue culture methods need to be established. In this study, detailed methods for introduction of mutations in tomato and Nicotiana benthamiana plants using the CRISPR/...

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Bibliografische gegevens
Gepubliceerd in:Plant Biotechnol (Tokyo)
Hoofdauteurs: Komatsu, Hiroki, Abdellatif, Islam M. Y., Yuan, Shaoze, Ono, Misaki, Nonaka, Satoko, Ezura, Hiroshi, Ariizumi, Tohru, Miura, Kenji
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Japanese Society for Plant Cell and Molecular Biology 2020
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Original Paper
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7434671/
https://ncbi.nlm.nih.gov/pubmed/32821229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5511/plantbiotechnology.20.0527b
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