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Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates

Transcription elongation rates influence RNA processing, but sequence-specific regulation is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping RNAPI by Miller chromatin spreads or UV crosslinking revealed 5′ enrichment and strikingly uneven local polymerase occu...

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Publicat a:Mol Cell
Autors principals: Turowski, Tomasz W., Petfalski, Elisabeth, Goddard, Benjamin D., French, Sarah L., Helwak, Aleksandra, Tollervey, David
Format: Artigo
Idioma:Inglês
Publicat: Cell Press 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7427326/
https://ncbi.nlm.nih.gov/pubmed/32585128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2020.06.002
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