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Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates

Transcription elongation rates influence RNA processing, but sequence-specific regulation is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping RNAPI by Miller chromatin spreads or UV crosslinking revealed 5′ enrichment and strikingly uneven local polymerase occu...

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Publicado en:Mol Cell
Autores principales: Turowski, Tomasz W., Petfalski, Elisabeth, Goddard, Benjamin D., French, Sarah L., Helwak, Aleksandra, Tollervey, David
Formato: Artigo
Lenguaje:Inglês
Publicado: Cell Press 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7427326/
https://ncbi.nlm.nih.gov/pubmed/32585128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2020.06.002
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