An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

Description complète

Enregistré dans:
Détails bibliographiques
Publié dans:Front Cell Dev Biol
Auteurs principaux: Javvaji, Pradeep K., Dhali, Arindam, Francis, Joseph R., Kolte, Atul P., Mech, Anjumoni, Roy, Sudhir C., Mishra, Ashish, Bhatta, Raghavendra
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2020
Sujets:
Cell and Developmental Biology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://ncbi.nlm.nih.gov/pubmed/32850864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2020.00764
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires