An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

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Detalhes bibliográficos
Publicado no:Front Cell Dev Biol
Main Authors: Javvaji, Pradeep K., Dhali, Arindam, Francis, Joseph R., Kolte, Atul P., Mech, Anjumoni, Roy, Sudhir C., Mishra, Ashish, Bhatta, Raghavendra
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Cell and Developmental Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://ncbi.nlm.nih.gov/pubmed/32850864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2020.00764
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