An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

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Опубликовано в: :Front Cell Dev Biol
Главные авторы: Javvaji, Pradeep K., Dhali, Arindam, Francis, Joseph R., Kolte, Atul P., Mech, Anjumoni, Roy, Sudhir C., Mishra, Ashish, Bhatta, Raghavendra
Формат: Artigo
Язык:Inglês
Опубликовано: Frontiers Media S.A. 2020
Предметы:
Cell and Developmental Biology
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://ncbi.nlm.nih.gov/pubmed/32850864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2020.00764
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