An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

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Publicado en:Front Cell Dev Biol
Autores principales: Javvaji, Pradeep K., Dhali, Arindam, Francis, Joseph R., Kolte, Atul P., Mech, Anjumoni, Roy, Sudhir C., Mishra, Ashish, Bhatta, Raghavendra
Formato: Artigo
Lenguaje:Inglês
Publicado: Frontiers Media S.A. 2020
Materias:
Cell and Developmental Biology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://ncbi.nlm.nih.gov/pubmed/32850864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2020.00764
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