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Generation of Differentially Modified Microtubules Using in Vitro Enzymatic Approaches

Tubulin, the building block of microtubules, is subject to chemically diverse and evolutionarily conserved post-translational modifications that mark microtubules for specific functions in the cell. Here we describe in vitro methods for generating homogenous acetylated, glutamylated or tyrosinated t...

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Bibliografske podrobnosti
izdano v:Methods Enzymol
Main Authors: Vemu, Annapurna, Garnham, Christopher P., Lee, Duck-Yeon, Roll-Mecak, Antonina
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7416453/
https://ncbi.nlm.nih.gov/pubmed/24630106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-397924-7.00009-1
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