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Low temperature bacterial expression of the neutral amino acid transporters SLC1A5 (ASCT2), and SLC6A19 (B0AT1)

It is well established that Escherichia coli represents a powerful tool for the over-expression of human proteins for structure/function studies. In many cases, such as for membrane transporters, the bacterial toxicity or the aggregation of the target protein hamper the expression limiting the appli...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Mol Biol Rep
Main Authors: Galluccio, Michele, Pantanella, Marta, Giudice, Deborah, Brescia, Stefania, Indiveri, Cesare
Format: Artigo
Jezik:Inglês
Izdano: Springer Netherlands 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7415195/
https://ncbi.nlm.nih.gov/pubmed/32772343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-020-05717-8
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