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FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca(2+). Here, we describe a microplate reader fluorescence resonance energy transfer (FRET) assay that use...
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| Published in: | Bio Protoc |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Bio-Protocol
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7409823/ https://ncbi.nlm.nih.gov/pubmed/32775537 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3641 |
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