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Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome

We previously demonstrated that the injection of pregnant wild-type female mice (carrying enhanced green fluorescent protein (EGFP)-expressing transgenic fetuses) at embryonic day (E) 12.5 with an all-in-one plasmid conferring the expression of both Cas9 and guide RNA (targeted to the EGFP cDNA) com...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Nakamura, Shingo, Ando, Naoko, Watanabe, Satoshi, Akasaka, Eri, Ishihara, Masayuki, Sato, Masahiro
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7409276/
https://ncbi.nlm.nih.gov/pubmed/32708213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9071744
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