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Cloning antibodies from single cells in pooled sequence libraries by selective PCR
Antibodies function by binding to antigens. Antibodies must be cloned and expressed to determine their binding characteristics, but current methods for high-throughput antibody sequencing yield antibody DNA pooled from many cells and do not readily permit cloning of antibodies from single B cells. W...
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| Udgivet i: | PLoS One |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7406036/ https://ncbi.nlm.nih.gov/pubmed/32756607 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0236477 |
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