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Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange
The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach for introducing large DNA constructs into the C. e...
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| Published in: | Genetics |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Genetics Society of America
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7404237/ https://ncbi.nlm.nih.gov/pubmed/32513816 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.120.303388 |
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