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Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange

The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach for introducing large DNA constructs into the C. e...

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Detalhes bibliográficos
Publicado no:Genetics
Autor principal: Nonet, Michael L.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7404237/
https://ncbi.nlm.nih.gov/pubmed/32513816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.120.303388
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